Abstract— Plant tissue culture has been used as a tool for crop improvement in many different ways. Such as somaclonal variation that occurred in many different crops. In this study a program for disease resistance was established in sesame using somaclonal variation. As resistance to Fos is very important so different kinds and concentrations of Plant Growth Regulators were tested for producing of plantlet regenerated from apical shoot explants.
The results showed that the combination of BA and NAA also BA and IAA with ABA could be used for regenerating sesame plantlets from apical shoots. The difference in BA concentrations had a positive effect on shoot and root regeneration and at least plant regeneration. So with combination of high level of BA and low level of NAA shooting from explants was dominant and with low level of BA and high level of NAA rooting was progressed. Regenerated plantlets and seeded planlets were compared for examining of resistance or susceptibility to Fos. The result showed that somaclonal variant resulted from regeneration of shoot and root of sesame could lead to producing resistant plantlets.
Keywords— fusarium, sesame, somaclonal variation.
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