Estimation of DNA Fragmentation, Mutagenicity and Biochemical Changes in Mice Exposed to Diazinon and Its Commercial Formulation

Abstract The aim of the present study was to determine the effect of organophosphorus (OP) insecticide diazinon (DZN) and its formulation (DZNF) in bone marrow and germ cells, DNA fragmentation and biochemical changes induced in Swiss albino male mice. The mice were randomly divided into 7 groups (10 mice each), the 1st group served as control, the 2nd, 3rd and 4th groups, as well, 5th, 6th, and 7th groups are treated with 1/10LD50 (6.5 mg/kg bw), 1/20 LD50 (13mg/kg bw) and 1/40 LD50 (26mg/kg bw) of DZNF and DZN i.p with single dose/week for 4 weeks, respectively. At the end of treatment all animals were sacrificed by cervical dislocation after 24 h of the last treatment. Bone marrow and spermatocyte cells were subjected to chromosomal analysis. As well, liver and brain tissues were collected from all animals for DNA fragmentation and biochemical analyses. Cytogenetic analysis revealeda significant increase (more than 2 folds) in structural aberrations (Chromatid and chromosomal gaps, breaks, deletions, centromeric attenuation and end to end)as well as numerical variations in DZNF treated groups than DZN treated groups in a dose dependent manner. A significant increase (about 3 folds) also was found in DZNF treated groups in structural and numerical aberrations of spermatocyte cells than DZN treated mice in a dose dependent manner. Levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA) and DNA fragmentation were significantly increased (about 2 fold) in mice exposed to DZNF than DZN exposed groups. In conclusion, our findings demonstrate that DZNF is more genotoxic than DZN as assessed by cytogenetic analysis of both somatic and germ cells of mice and had adverse effects on DNA and biochemical parameters. These results suggested that the effects of DZNF and DZN are dose dependent and the treatment with DZNF is more hazardous and toxic than DZN and it is important to avoid toxicities induced by organophosphate insecticides, take a high level of caution and minimize its agricultural and household uses.

Keywords— Diazinon, formulation, mutagenicity, DNA fragmentation, biochemical changes, mice.

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International Journal of Environmental and Agriculture Research (IJOEAR)

ISSN: 2454-1850

Digital Object Identifier DOI: 10.25125/agriculture-journal

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