Abstract— In vitro efficacy of fungus and bacteria isolated from mushroom substrates were evaluated against selected pathogens (Fusarium oxysporum, Sclerotium rolfsii, and Colletotrichum corchori). There were four fungi Aspergillus flavus, Aspergillus niger, Trichoderma harzianum, and Penicilliumsp and four bacteria Bacillus subtilis, Bacillus cereus, Pseudomonas sp. and Paenibacillus polymyxa isolated from substrate. Different biochemical and pathogenicity test were performed to confirm their species. In in-vitro dual culture method T. harzianum showed the highest inhibition in case of Fusarium oxysporum (70.26%) followed by Sclerotium rolfsii (59.94%) and Colletotrichum corchori (57.02%) whereas Penicillium sp showed the least significant result against these pathogens. To inhibit the growth of Sclerotium rolfsii and F. oxysporum. Pseudomonas spp. was significant around 72.19% and 62.62% respectively. On a comparative study among the four isolated bacteria and fungi Pseudomonas sp. and T. harzianum showed the best significant antagonistic activity against all the selected plant pathogenic fungi.
Keywords— In vitro, isolated, mushroom substrates, dual culture, pathogenic fungi.
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