Generation of Hydrogen Gas from Crude Glycerol by Purple Non-Sulfur Photo Fermentative Bacteria, Rhodobacter Meghalophilus

Abstract As the world is progressing faster with new technological innovations, the need and demand for energy is also constantly expanding. In the light of conventionally available fossil fuel reserves being exhausted extensively that has left a very deep scar on environment, the urge for alternative environment friendly energy source is the need for energy sustenance. Hydrogen gas is distinct for its high calorific value, clean fuel characteristic and suitability for wide applications. Chemical method likes steam reforming, coal gasification are established technologies available for industrial hydrogen needs but are high in terms of cost and energy input. Biological methods are promising routes for hydrogen gas generation as they can be cost effective and use a variety of organic materials as substrates. The current study is focused on generation of hydrogen gas using Rhodobacter meghalophilus, a mesophilic, and purple non sulfur photo fermentative bacteria. Crude glycerol, byproduct from biodiesel plants is used as carbon substrate because of its rich organic content. Experiments were carried out to study the effect of process parameters viz. volume of crude glycerol, pH and light intensity on generation of hydrogen gas. Crude glycerol in the media was varied from 5 – 15% (v/v), pH between 5.8 – 8.3 and the light intensity at 500, 1000 and 2000 Lx respectively. At 15% (v/v) of crude glycerol, pH of 7.8 and 1000 Lx, the volume of hydrogen gas obtained was 490 ml/L with the substrate to hydrogen gas conversion rate as 0.012 mol/ mol of crude glycerol with light conversion efficiency of 1.16%.

Keywords— Bio hydrogen, Clean energy, Crude glycerol, Photo fermentation, Rhodobacter meghalophilus.

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Engineering Journal: Generation of Hydrogen Gas from Crude Glycerol by Purple Non-Sulfur Photo Fermentative Bacteria, Rhodobacter Meghalophilus

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