Isolation and Identification of Mycoplasma Species in Dogs

Abstract Mycoplasmas can be associated with several canine health issues, mainly when dogs do not respond to antimicrobial treatment usually aimed at bacterial infections. Different mycoplasma species can be found in both healthy and sick animals; however, the following subjects have yet to be fully understood: The role played by mycoplasmas in canine habitats and the various diseases caused by them. The aim of the present study is to assess the presence of mycoplasma in dog samples at NUDMIC/UFF, RJ, Brazil, over a timeframe of ten years.  Out of all assessed dogs, 9.67% (15/155) had respiratory symptoms, whereas the rest of them were asymptomatic. Moreover, 29.96% of the cultured samples (77/257) were positive for mycoplasmas. Typical colonies of said samples were divided into 42.86% (33/77) of oropharynx samples, 51.95% (40/77) of urogenital samples and 5.19% (4/77) of samples from other sources. Species Mycoplasma canis, Mycoplasma edwardii and Mycoplasma cynos were identified by PCR and/or immunoperoxidase. The most common species was M. canis. M. cynos was found in a dog with signs of respiratory disease. Despite the recent improvement in early identification and the biomolecular knowledge surrounding canine mycoplasma, the etiopathogenesis of canine mycoplasmosis remains uncertain..

Keywords Diseases, dogs, isolation, Mycoplasma, PCR.

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Agriculture Journal: Isolation and Identification of Mycoplasma Species in Dogs

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