Abstract— Qualitative and quantitative analysis of selected mycotoxins has been performed in extracts of Conidiobolus coronatus pathogenic fungus cultivated under optimal and stress conditions. Furthermore, the analyses of these compounds in post-incubation filtrates were done. For identification purposes the analytical method allows identification and quantitation of selected mycotoxins including beauvericin , fumonisin B1, enniatin A and B and destruxin A based on high performance liquid chromatography coupled with tandem mass spectrometry was developed. Only beauvericin was detected in very low amounts in C. coronatus mycelium extract cultivated under optimal condition. In the extract of C. coronatus mycelium grown on LB 12.3 ± 0.1 µg/g of beauvericin was determined, while in the extract of C. coronatus mycelium grown on MM medium beauvericin content was lower and amounted 4.6 ± 0.1 µg/g. Also the presence of beauvericin was confirmed in postincubaction filtrate extract (MM). The content of this compound was 2.2 ± 0.1 µg/g. In other extracts beauvericin was not detected. In addition, in the tested extracts other compounds were not detected.
Keywords— Mycotoxin Production, Conidiobolus, quantitative analysis.
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