Abstract— It is clear that, beneficial species of Citrus need to improvement especially by new methods due to limitations of conventional methods. New methods like biotechnology and gene transfer need to establishment of regeneration plants by tissue culture. Shoot proliferation in Citrus is easy but rooting of proliferated micro shoots has been discussed in various articles. The goal of this study is presentation of a proper method for rooting of micro shoots in Citrus by manipulation of media content. Hypocotyl segments of Citrus aurantifolia from 45 day old seedlings and 0.5 – 0.7 cm in length were cultured on MS media supplemented with different kinds and concentrations of plant hormones suitable for shooting and rooting such as BA, IBA and NAA alone or together. 1 mg/l BA and 1.5 mg/l NAA on MS media was the best treatment for shooting and rooting respectively. In this study we can overcome one of the most important problems of establishing regeneration system in Citrus and opening the way for biotechnology and gene transfer for this important and economic plant.
Keywords— Citrus aurantifolia, gene transfer, rooting, shooting.
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