Abstract— Labisia pumila or commonly known as kacip fatimah is one of the popular medicinal plant in Malaysia. The constituents of this plant have been reported to possess anti cancer, antioxidant, anti-obesity and anti-inflammatory properties. The growth and production of L. pumila is greatly influenced by the environmental condition such as shade, humidity and growing media. In this study, the survivality of L. pumila var. alata (KFeFRIM01) plantlets derived from tissue culture technique using temporary immersion system were analyzed during pre and post acclimatization process. The plantlets were pre acclimatize in different potting media namely 100% sand and 100% jiffy and grown in plastic growth chamber with different percentage of shade (0%, 50% and 70%). After a month, the plants were transferred to the nursery for post acclimatization. Three experiments were conducted to evaluate the survivality of plants placed under different percentage of shade (70% and 50%), suitable growing media and watering requirement per day. The growth of the plants such as plant height, number of leaves, leaf length and leaf width were recorded during the acclimatization process. The analysis of variance (ANOVA) was conducted to evaluate the survivality and growth of this plant during the acclimatization process. KFeFRIM01 was found to grow better in 0% shade compared to 50% shade and 70% shade. However, there were no significant difference recorded in the usage of two potting medium (100% sand and 100% jiffy) on the growth of KFeFRIM01 during pre acclimatization process. While in post acclimatization, KFeFRIM01 placed under 50% shade produce better growth in term of leaves number (8.44a ± 0.20), leaf length (6.13a ± 0.14) and leaf width (3.17a ± 0.06) compared to 70% shade. KFeFRIM01 plants planted in treatment 1-top soil: leaf compost: sand (2:3:1) gave the highest growth performance in all parameter measured. Whereas, KFeFRIM01 plants that watered twice per day (9AM & 4PM) have greater plant height (8.83a ± 0.34) and leaves number (7.53a ± 0.39) compared to plants watered once per day. The findings from this study are essential for mass production of L. pumila using tissue culture technology in future as a successful protocol for acclimatization of this plants obtained.
Keywords— adaptation, environmental condition, plant growth, survivality, clonal propagation.
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