Cost effective production of Bacillus thuringiensis aizawai and their application against Spodoptera litura

Abstract Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) was prevalent in many species of aquatic plants grown in Green Farms. This study was carried out to understand the production of Bacillus thuringiensis aizawai (Bta) using cost effective method to manage the pest biologically. Bta is being widely used in pest control programs. However, the production of this Bta is expensive due to the high cost of the production medium. In this study, an attempt has made to develop a cost-effective medium, based on a locally available raw materials namely coconut water which is available in plenty as waste product from coconut oil industry, coconut poonac, rice bran and coir dust. A standard conventional Luria Bertani medium was included in the assay for comparison. Media were assessed for the growth, sporulation and production of insecticidal properties of Bta. Coconut poonac extract and coconut water media produced higher spores than compare with conventional LB medium. Maximum spore count of 25.0×1013 spores/mL was obtained with a 72 h old culture of this bacterium grown in coconut poonac extract. Larvicidal activity (LC50) of 8 ×106 spores/mL (coconut poonac extract) against early second-instar larvae of S.litura were obtained. This is almost similar to that obtained with LB (9 × 106 spores/mL) medium. Hence, coconut based culture medium is economical for the production of Bta and compared favorably with the standard. Cost-effective analyses have revealed that production of Bta from test media is highly economical. The cost of production of Bta with local media was significantly reduced by 88-293 fold. The use of nonconventional sources has yielded a new knowledge in this area as the process development aspects of small scale production have been neglected as an area of research. These studies are very important from the point of media optimization for economic production of Bta based agents in pest management programs.

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Cost effective production of Bacillus thuringiensis aizawai and their application against Spodoptera litura

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