Abstract— The present study was conducted at Banana Tissue Culture lab of Regional Plant Resource Centre, Bhubaneswar, Odisha to obtain a standardized micropropagation protocol supplemented with different concentrations and combinations of cytokinins and auxins for Musa cv. Yangambi Km-5 (AAA) variety. Data collected for in vitro culture consists of the following parameters: days for bud initiation, rate of shoot proliferation (%), growth value (gm) and number of multiple shoot buds during multiplication stage. Remarkable effects of Cytokinins and Auxins were observed in Yangambi Km-5. Out of various treatments, best concentration for multiple shoot in short period of time for Yangambi Km-5 was found in mediums 3 mg/l BAP + 0.5 mg/l IAA + 0.25 mg/l NAA + 100 mg/l ADS and 1.5 mg/l BAP + 1.5 mg/l KN + 1 mg/l IAA + 0.25 mg/l NAA + 100 mg/l ADS. Maximum growth value, rate of proliferation and number of shoot buds was obtained from explants culture medium 3 mg/l BAP + 0.5 mg/l IAA + 0.25 mg/l NAA + 100 mg/l ADS.
Keywords— Micropropagation, growth regulators, Yangambi Km-5, Musa spp.
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