Effects of curing systems and light units on cytotoxicity of dental composites

Abstract— The objective of this study was to examine and compare the cytotoxicity behavior of commercial two restorative (light-cured) and three adhesive composites (dual-cured) polymerized by using two different light curing units (LCU). Commercial composites Filtek Z250, Filtek Supreme XT, Rely X Arc, Rely X U100 and Variolink II were polymerized using different light densities of halogen (H) and Light Emitting Diod (LED) curing units. After the polymerization process samples sterilized under UV light for 15 minutes. Dulbecco’s Modified Eagle’s Minimal Essential Medium (DMEM) containing 200 µL of serum was placed in 96 well cell plates and samples were added in the wells. They were incubated 5% CO2 incubator for 48 hours at 37°C.  Sample surface area/solution volume was adjusted to 2.5 cm2/ml. Cytotoxicity of samples was examined by the extraction method and the results were evaluated using the MTS test. The extracts of the samples were collected for 24 hours and incubated in L-929 mouse fibroblast cells (MFCs). The data was analyzed with the SPSS statistics program. Samples polymerized by H light source were generally cytotoxic than the samples polymerized by LED light source. Rely X Arc in dual-cure system is the most biocompatible material and Variolink II-LED combination is the most cytotoxic one. Furthermore, there was no statistically significant difference between the cytotoxicity levels of composites using H and LED light sources (p>0.05).This study showed that the curing treatment used power density LED affects biocompatibility positively and nano-structures increase the biocompatibility.

Keywords— Cytotoxicity, dental composites, light curing, dual curing, halogen, LED, light curing units.

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Dynamic analyses of a flat plate and a beam subjected to a moving load

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