Abstract— Supplementation is one of the most relevant procedures for mushroom growth modulation. Little is known about the influence of supplementation on the concentrations of metabolites with potential health benefits. Experiments on compost require long cultivation times. Similar composition has been detected in fruiting bodies and mycelia. Therefore, the mycelial composition can be assumed to be similar to that of the fruiting bodies. This study examines the effect of supplementing a minimal defined growth medium with components derived from olive oil industry subproducts on A. bisporus mycelial composition, primarily ergosterol, when grown on minimal defined liquid and solid media in an attempt to obtain a higher concentration of ergosterol (pro-vitamin D2).
bisporus supplemented with alperujo meal (ALPM) and olive leaf meal (OLM) led to higher ergosterol content than that of the fungi grown in non-supplemented media (5.64±0.47, 6.60±0.86 and 4.08±0.53 mg/g p.s. in MDLm and 5.36±0.39, 6.79±0.41 and 4.22±0.43 mg/g p.s. in MDSm). Western blotting was used to validate the cultivation results. Three proteins (ERG2, ERG6, and EGR11) involved in the ergosterol biosynthetic pathway were significantly upregulated, indicating the importance of supplementation to ergosterol biosynthesis.
This report represents the first comprehensive study on the protein expression profiling of supplementation studies directed to improve metabolites with potential health benefits in A. bisporus. It provides new insights and a better understanding of the development of cultivation processes directed to increase ergosterol biosynthesis. These results could be used to obtain mycelia with higher vitamin D2 content after irradiation with UVB light.
Keywords— Agaricus bisporus, cultivation medium supplementation, ergosterol, olive leaf meal, two-phase pomace.
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