Abstract— Strong oxidising agents for bleaching and chemical treatments for regeneration of dry and damaged hair are most common factors that cause changes in protein structure and composition. Composition changes in fibrillar proteins – α keratins and globular proteins – KAP’s (keratin-associated proteins) were investigated by protein analysis of the hair fiber using SDS-PAGE. The most significant changes in obtained electrophoregrams from SDS-PAGE analysis of bleached hair were observed in matrix protein fractions (KAP’s), which contain a high concentration of cystine. Constructed electrophoregram from SDS-PAGE gel of hair treated with protein treatment indicates a new protein fraction with a molecular weight of about 2000 Da, probably due on hydrolyzed keratin as an ingredient of protein treatments.
Keywords— bleaching, hair protein treatments, SDS-PAGE.
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