Abstract— β-1,3 glucanases are semi-constitutive hydrolytic enzymes that can degrade glucan molecules embedded in the cell wall components of cereals and some species of fungi resulted in production of D-glucose. This enzyme has a great potential and interest in biotechnology, agricultural and also industrial field. However, there is little reports on the production of β-1,3 glucanase by Penicillium oxalicum. Therefore, the cultural conditions which stimulate in vitro production of β-1,3 glucanase enzyme by P. oxalicum T3.3 and characterization of β-1,3 glucanase enzyme activity were determined.Various parameters such as different types of carbon and nitrogen sources, initial pH medium, agitation speed and surfactants were investigated. The optimization was carried out by varying and optimizing one variable at a time. The highest production of β-1,3 glucanase activity of 84.73 U/mL was obtained using seaweed Undaria pinnatifida as substrate at concentration of 1% (w/v), peptone and yeast extract as nitrogen source at 0.3% and 0.2% respectively, initial medium pH 5, agitation speed at 200 rpm and with addition of sodium dodecyl sulfate as surfactant. Under these conditions, β-1,3 glucanase activity increased by 38.6%. Enzyme characterization was also performed which indicated that this enzyme is thermostable and showed optimum activity at 50°C, pH 5 and can retained its activity around 80% up to 4 h at this condition.The optimization of β-1,3 glucanase production by P.oxalicum required adjustment of different types of carbon and nitrogen sources, initial pH medium, agitation speed and surfactants. This enzyme characterization has revealed its great potential towards detergent, beer and food fermentation industries whose manufacturing conditions are largely acidic.
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