Flow Injection Analysis of Indigo Carmine Using Green Coconut (Cocos Nucifera L.) Fiber as a Bioadsorbent

Abstract This paper deals with a procedure for preconcentration and determination of indigo carmine in textile industrial effluent. The product of Indigo blue sulphonation, 5,5´-disulfonic indigotin (5,5´-DI) aqueous solution was run through a mini-column packed with green coconut fiber (Cocos Nucifera L.) in a flow injection system. The 5,5´-DI was preconcentrated on the sorbent at pH 2 and sample flow rate 3.9 mL min-1 and eluted by HCl 0.1 mol L-1 to be detected by UV-vis spectrophotometry at 612 nm. An increase of 18.7% in the indigo recovery was observed with fiber washed with albumin and 11.7% in fiber washed with NaOH. A linear response was observed within the range 0.157 to 75.2 µmol L-1, with detection limit, coefficient of variation and sampling rate estimated as 0.0376 µmol L-1 (95% confidence level), 4.5% (n = 21) and 101 determinations per hour, respectively. The enrichment factor achieved was 1.64. The proposed method was successfully applied to the determination of 5,5´-disulfonic indigotin in textile industrial effluents.

Keywords Adsorption, flow-analysis, green coconut, solid-phase extraction, spectrophotometry.

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Flow Injection Analysis of Indigo Carmine Using Green Coconut

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