Abstract— Practical immunoassay chip devices are high-priority needs in point-of-care testing (POCT) for rapid diagnoses. Compared to conventional POCT microchip devices, our report describes a manufacturing process involving laser ablation, inkjet deposition, and cover film sealing that is superior to that used for practical mass production of microchip devices, and produces devices capable of sensitive and reproducible measurements using a capillary-flow-driven system at a practical point-of-care setting. To promote sensitivity and reproducibility, circular islands surrounded by 10-µm-deep grooves were prepared to provide uniformity of printed antibody spots on a capillary flow immunoassay chip. The island surface enabled dense antibody fixation due to droplet surface tension as well as antibody determination by enzyme linked immunosorbent assay (ELISA), which demonstrated greater sensitivity than that of a device using a non-island surface. The luminescence intensity of the spots of the carboxyterminal propeptide of type I procollagen (PICP) exhibited a good linear relation with PICP concentration in the range 0-600 ng·mL-1, which is suitable for clinical estimation in blood.
Keywords— Capillary driven flow, Sensing Chip, grooved Island, immunoassay chip, ELISA.
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