Abstract— The main aim of the present study was to evaluate the hepatoprotective and stress-reducing effects of Moringaoleifera extract against A. hydrophila infection and transportation- induced stress in Nile tilapia, Oreochromisniloticus fingerlings. Fish were fed diets representing different supplementation levels of Moringaoleifera leaf extract. The graded levels of M. oleifera leaf extract were 0.00g (control), 0.05g, 0.10g, 0.15g, 0.20g, 0.25g per 100g for each diet. After six weeks of the feeding trial, fish previously fed each experimental diet were exposed to pathogenic strain of Aeromonashydrophila at a concentration of 9.3 × 105 CFU /mL. After bath exposure, fish from each dietary treatment was placed into the aquaria culture system. They were fed their respective diets at 5% body weight twice daily, and mortality was monitored for the remaining 4 weeks of the feeding trial. After the feeding trial, fish previously fed each experimental diet were kept in plastic tanks for a 2-hour journey. Blood and liver samples were collected for hepatocellular assessments (Aspartate transaminase (AST), Alanine transaminase (ALT), Lactate dehydrogenase (LDH) and Malate dehydrogenase (MDH) tests) and stress biomarkers (survival, cortisol and glucose). Results showed that the increases of the AST, ALT, LDH, MDH, cortisol and glucose induced by stressors were significantly reduced (P< 0.05) by supplementing the fish with M. oleifera leaf extract in the diets. Based on the result of this study, a dose of 0.10g/100g dietary Moringa leaf supplementation was sufficient as a hepatoprotective and stress reducing agent in Nile tilapia, O. niloticus.
Keywords— Moringaoleifera, hepatoprotective,stress-reducing, Oreochromisniloticus, supplementation.
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