Abstract— Mycoplasma hyopneumoniae is a fastidious bacterium, an important member of swine respiratory disease complex, like Porcine Enzootic Pneumonia, affecting the non-specific defense mechanism of the respiratory tract, high mucociliary system, predisposing the pigs to secondary pathogens. The objective of this study is to implement precise diagnostic techniques for identification of Mycoplasma hyopneumoniae. 19 swine lungs fragments were collected from slaughterhouses and submitted by histopathological techniques. The presence of mucocellular exudate in 78.94% of the samples was observed in the bronchi and bronchioles, absence of eyelashes in 63.15% and lymphoid tissue hyperplasia associated with the bronchus in 42.10%. In pulmonary parenchyma, thickening of alveolar wall and interstitial bronchopneumonia were observed in 68.42%, hemorrhage in 47.36%, which 36.84% had hemosiderin and 15.78% lung consolidation. The presence of mycoplasma by the negative staining technique was identified in all samples, also the labeling of epitopes by colloidal gold immunostaining, using monoclonal antibody. In immunohistochemistry techniques and in situ hybridization, the labeled epitope and genome were observed confirming the presence of Mycoplasma hyopneumoniae in the State of São Paulo. Therefore, the bronchial epithelium is the best tissue to collect the sample for an accurate diagnosis and the best method of diagnosis is the negative staining technique for screening and colloidal gold immunocytochemistry techniques to identify Mycoplasma species.
Keywords—Mycoplasma hyopneumoniae, Porcine Enzootic Pneumonia, Immunochistochemistry and Hybridization.
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