Influence of Plant Growth Regulators and Explant Type on Multiple Shoot Induction and Somatic Embryogenesis in Sesame (Sesamum indicum L.)

Abstract Sesamum indicum L. is used as an important oil crop in the world. For establishing of a simple and a rapid system for in vitro culture of sesame shoot tips and hypocotyls explants were cultured on MS media with differtent combinations and concentrations of PGRs. On medium with 5 mgl-1BA plus 1 mgl-1 IAA and  1 mgl-1 ABA mutliple shoot induction on explants was occurred. Multiple shoot indution on medium supplemented with 2 mgl-1BA and 0.3 mgl-1 NAA  happen with a short phase of callusing. Also MS medium with 3 mgl-1 2,4-D and 0.5 mgl-1 kinetin was a suitable medium for inducing of somatic embryogenic calli with the frequency of sub-culturing potential. Regenerated shoots on both media rooted on MS medium plus 1.5 mgl-1 NAA and 0.03 mgl-1 BA and whole regenerated plantlets formed.

Abbreviations: 2,4-D – 2,4-dichlorophenoxy acetic acid; ABA – Abscisic acid;  BA – 6 benzylaminopurine; IAA – Indole – 3 – acetic acid; IBA – Indole – 3 – butyric acid; Kinetin – n6 – Furfyrylaminopurine; MS – Murashige and Skoog medium; NAA – Naphtalene acetic acid; PGRs – Plants Growth Regulators.

Key words: adventitious shoots, embryogenic calli, sesame.

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