Abstract— Sesamum indicum L. is used as an important oil crop in the world. For establishing of a simple and a rapid system for in vitro culture of sesame shoot tips and hypocotyls explants were cultured on MS media with differtent combinations and concentrations of PGRs. On medium with 5 mgl-1BA plus 1 mgl-1 IAA and 1 mgl-1 ABA mutliple shoot induction on explants was occurred. Multiple shoot indution on medium supplemented with 2 mgl-1BA and 0.3 mgl-1 NAA happen with a short phase of callusing. Also MS medium with 3 mgl-1 2,4-D and 0.5 mgl-1 kinetin was a suitable medium for inducing of somatic embryogenic calli with the frequency of sub-culturing potential. Regenerated shoots on both media rooted on MS medium plus 1.5 mgl-1 NAA and 0.03 mgl-1 BA and whole regenerated plantlets formed.
Abbreviations: 2,4-D – 2,4-dichlorophenoxy acetic acid; ABA – Abscisic acid; BA – 6 benzylaminopurine; IAA – Indole – 3 – acetic acid; IBA – Indole – 3 – butyric acid; Kinetin – n6 – Furfyrylaminopurine; MS – Murashige and Skoog medium; NAA – Naphtalene acetic acid; PGRs – Plants Growth Regulators.
Key words: adventitious shoots, embryogenic calli, sesame.
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