Abstract—Fructosyltransferases (FTase, E.C. 2.4.1.9) are enzymes that catalyze transfructosylation reactions obtaining, as final product, fructose oligomers. In terms of industrial production, the use of microbial enzymes is interesting, especially those produced by Aspergillus sp. The aim of the work was to study the production of FTase by submerged fermentation and evaluation of pH and temperature effects on fructosyltransferase activity. The enzyme was produced by Aspergillus oryzae IPT 301 in a 10-liter bioreactor with growth medium containing sucrose as main carbon source. The assay was performed at 800 rpm, 30 °C, 0.75 vvm and pH 4.5. Transfructosylation (At) and hydrolytic (Ah) activities were determined in the temperature range from 35 to 65 °C and pH range from 3.5 to 6.0. It was observed that mycelial is increases with temperature, holding the maximum value at 50 – 65 ºC, while the optimum pH value were 5.0. The optimum temperature for extracellular At ranged from 55 to 65 °C and the optimum pH ranged from 5.0 to 6.0. Furthermore, the optimum temperature for mycelial Ah was 65 °C and optimum pH 4.5 – 5.0. For extracellular Ah, the optimum temperature and optimum pH were 55 to 65 °C and 3.5 to 6.0, respectively.
Keywords—Aspergillus oryzae, Bioreactor, Fructooligosaccharides, Fructosyltransferase.
Click here to Download Full Paper
AD Publications is a rapidly growing academic publisher in the fields of Engineering, Medical-Health, Environmental Science and Agriculture Research. AD Publications is a registered organization broad-based open access and publishes most exciting researches with respect to the subjects of our journals. The Journals is being indexed and abstracted by all major global current awareness and alerting services.
The organization aims at undertaking, co- coordinating and promoting research and development. It provides professional and academic guidance in the field of basic education, Higher Education as well in the Technical Education. Our Aims is to Promote and support, High Quality basic, Scientific Research and development in fields of Engineering, Medical-Health, Environmental Science and Agriculture Research and to Generate Public awareness, provide advice to scholar’s researchers and communicate research outcomes.
Some Important Links About Research Journal
International Journal
Agriculture Journal
Medical Journal
Environmental Journal
Engineering Journal