Abstract— Pencillium citrinum whole cell was cultivated and immobilized in a low cost support aiming to be used as an active and stable biocatalyst for modification of oil and fats. PUF (polyurethane foam) coated in 6mm was used as support and the immobilization occurred as a natural consequence of cell growth. Olive oil was the mycelium-bound lipase inductor and the production was optimized by statistical analysis of pH and temperature effects in culture broth. Immobilized whole cell was characterized as a natural immobilized lipase and all the assays were made using olive oil hydrolysis. The mycelium-bound lipase production was improved by adjustment the culture broth to pH 7.5 and 35°C of incubation temperature. The SEM micrographs showed the entangled cells morphology and a high adhesion in support matrix. Biochemical characterization revealed maximum values of lipase activity in pH 8 and 40°C and a half-life time at 60°C was 2.2h. Results from kinetics study indicated the biocatalyst follow the Michaelis-Menten kinetic. The potential catalytic of immobilized whole cells was assessed in soybean oil hydrolysis and 55.7% of degree of hydrolysis was attained in 12h.
Keywords— whole cell, mycelium-bound lipase, immobilization, hydrolysis, oil.
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