Optimization of Storage Methods of Cowpea (Vigna Unguiculata L. Walp) Bagged Pics Containing Biopesticide (Lippia Multiflora) By Central Composite Experimental Design in COTE D’IVOIRE

Abstract The dried leaves of Lippia multiflora were tested for the efficiency on the stored cowpea kernels in PICS bags. A central composite design with five levels represented by two factors affecting the beans storage was used for control the evolution of merchantability (weight loss) and health (AFB1, OTA and Aw) quality during the storage. The factors were: storage time (1 to 8 months) and quantity of biopesticides (0 to 5% of the container mass). Results showed that it is possible to assess ideal conditions to keep the cowpea kernel merchantability and health qualities during storage. The quality of the kernels maintained for a concentration in biopesticide greater than or equal to 1,26% during 8 months. In the planned optimal conditions, the experimental values were 3.50±0.50%, 1.48±0.3 µg/kg, 4.54±0.02 µg/kg and 0.71±0.03 for weight losses, aflatoxin B1, ochratoxin A and water activity (Aw) respectively. These values of weight losses, mycotoxins levels and were substantially equal to those predicted by the experimental model.

Keywords Cowpea, PICS bags, biopesticides, central composite design, safety quality.

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