Abstract— An efficient and relatively simple regeneration system was developed for an elite cultivar of Chinese Jujube, a perennial tree, by culturing young twig segments as explants from 8-15 year old trees. The twig segments were disinfected by submerging them in 1% sodium hypochlorite (NaOCl) for 15 min with 3 min vacuum. Calli developed from both ends of the twig segments on half-strength MS medium supplemented with sucrose and BA or BA and NAA in combination. The frequency of shoot formation from calli was higher than 80% when the explants were placed on the half – strength MS medium supplemented with BA (2.581 μM) and NAA (2.685 μM). Roots were produced from adventitious buds for 90% of the regenerated shoots when they were placed on the MS medium supplemented with 4.920 μM IBA and 5.708 μM IAA. After transplanting to soil, 82% of the regenerated seedlings survived when they were covered with glass containers to maintain humidity. The results suggest that Chinese jujube can be reproduced and multiplied using organogenesis with the appropriate explant and culture medium.
Keywords— explants, regeneration, vacuum.
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