Osteoclastic resorption and osteoinduction in the highly purified B-tricalcium phosphate implanted in the rat subcutaneous tissue are promoted by autologous bone marrow cells.

Abstract Sequential cellular events after highly purified b-tricalcium phosphate (b-TCP) implantation with or without rat bone marrow (BM) cells were investigated. b-TCP disks were soaked with BM cells harvested from femora of syngeneic rats, and were implanted into subcutis of the eight-week-old rats. Specimens were harvested in the intended stage and histological examinations were performed.

In BM-treated group, new bone formation was detected and intense signals of α1 chain of typeⅠ procollagen mRNA were expressed in osteoblasts after day 14. On day 56, new bone never degenerated and normal bone marrow tissue was observed. TRAP positive-multinucleated cells appeared after day 7 and cathepsinK-positive osteoclasts appeared after day 14 with directly attached to b-TCP. Conversely, in BM non-treated group, new bone formation was never observed even on day 56 and neither TRAP nor cathepsinK positive-osteoclast was detected in the whole stage. Quantitative analysis showed that b-TCP area decreased time-dependently in BM-treated group, but not in BM non-treated group. In conclusion, these findings indicate that b-TCP loaded with BM cells has osteoinductive ability. b-TCP is resorbed mainly by osteoclasts and this event promotes ectopic bone formation. BM cells play an important role in osteoinduction and osteoclast differentiation and function.

Keywords Bone marrow cells; Osteoinduction; Osteoclasts; b-tricalcium phosphate.

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