Redox Mediated Decolorization and Detoxification of Direct Blue 80 by Partially Purified Ginger (Zingiber officinale) Peroxidase

Abstract Textile industries are releasing a large number of toxic synthetic dyes into waste waters. Hence, the removal of such compounds from environment prior to their final disposal is necessary. In the present study, potential use of ginger (Zingiber officinale) peroxidase in decolorization and detoxification of direct blue 80 has been investigated. It was found that only 0.166 U/ml of ginger peroxidase was sufficient for maximum decolorization of dye (25 mg/L). H2O2 was required in low concentration (0.3 mM) in the presence of 0.6 mM 1-hydroxybenzotriazole. Direct blue 80 was also successfully removed in stirred batch process.  It was observed that ginger peroxidase was highly stable over a wide range of pH and temperatures. Km and Vmax of the enzyme for direct blue 80 was found to be 27.8 mg/L and 2.09 mg/L/min, respectively. In UV-visible spectral analysis a sharp decline in peak was observed for the treated direct blue 80 which substantiates the breakdown of chromophore group of dye. Genotoxicity assessment by comet assay and chromosomal aberration test confirmed that the direct blue 80 was successfully detoxified by ginger peroxidase. Other direct and acid dyes were also treated either as a single or a mixture of different dyes and it was observed that these dyes were also decolorized significantly under similar experimental conditions. Our study suggests that this enzyme-redox mediator system constitutes a cost effective model which can decolorize the industrial textile effluents and also can reduce the toxic load of environment.

Keywords Decolorization; peroxidase, 1-hydroxybenzotriazole, Zingiber officinale

Abbreviations: DB 80, direct blue 80; DY 4, direct yellow 4; DY 50, direct yellow 50; DR 23, direct red 23; AB 1, acid black 1; AB 210, acid black 210; AY 42, acid yellow 42; GP, ginger peroxidase; HOBT, 1-hydroxybenzotriazole; PBS, phosphate buffer saline; MI, mitotic index; MMS, methyl methane sulphonate; CA, chromosomal aberrations.

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