Abstract— This study was designed to investigate the presence of Fusarium species in Mexican corn. Maize samples from 26 States were analyzed. Corn kernels were cultivated following a sequence of cultivation methods until obtaining spores which were transferred to carnation leaf agar medium. Taxonomic identification of fungi was carried out by microscopic examination. To evaluate the in vitro production of fumonisin B1, it was experimentally induced in un-contaminated maize. The quantitative determination of fumonisin B1 in the maize samples was performed by thin layer chromatography. Quality control and sensitivity were established using a standard solution of commercial origin whose purity was corroborated by both thin-layer chromatography and high-performance liquid chromatography.
Thirty-eight strains were isolated; 29 corresponded to Fusarium verticillioides and 9 to Fusarium subglutinans. Strains of Fusarium verticillioides exhibited a variable behavior in fumonisin B1 production. 4 strains produced fumonisin B1 in a range of 3.12 to 6.57 ppm.
In conclusion, two species of Fusarium; Fusarium verticillioides and Fusarium subglutinans were found in maize from 26 States of Mexico, their distribution is regionalized. Strains found in five States produced fumonisin B1 in concentrations that can be considered clinically relevant.
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