Abstract— Screening of 20 maize genotypes against Southern Leaf Blight of maize (Bipolaris maydis (Nisik) Shoemaker) was carried out in RCBD in National Maize Research Program, Rampur, Nepal during June to September, 2015. The area of research field was 315 m2. Each genotype had two rows per plot of 7.5m2 with two replication. Disease incidence was taken for three times at 43, 53 and 63 DAS. Disease scoring was done as percentage of leaf area infected on individual plant at 7 days interval starting from 58 days after sowing for 5 times and disease severity and mean AUDPC was calculated. Also the yield was calculated. Shade house experiment was carried out in a Completely Randomized Design with 3 replication on 20 genotypes by artificial inoculation at 3-4 leaf stage with a pure culture suspension of Bipolaris maydis (4×104conidia per ml) .and disease incidence and survival days of plant were recorded. Among the genotypes disease severity varied in the field. Highly significant differences were observed among the genotypes for Southern Leaf Blight severity, Area Under Disease Progress Curve and grain yield. Genotypes with mean AUDPC values from 200-250, 250-300 and above 300 were categorized as moderately resistant, moderately susceptible and susceptible. Disease severity was highest on genotype Rampur 24, 07 SADVI and lowest on BGBYPOP, RML-32/RM-17 and RAMS03F08. Highest maize yield (4.44 ton/ha) was recorded on RML-32/RML-17 and least (1.41 ton/ha) was obtained in ZM-627.In shade house, Rampur-24 followed by 07 SADVI, Rampur 27 died earlier and RML-32/RML-17 and BGBYPOP survived to the longest periods after inoculation. Disease Susceptibility pattern was similar in both field and shade house condition. The genotypes RML-32/RML-17, BGBYPOP, RAMS03F08 and TLBRS07F16 could be developed as resistant varieties to Southern Leaf Blight of maize and also as high yielders during summer under Chitwan and similar conditions.
Keywords— AUDPC, Bipolaris maydis, disease scoring, inoculation, resistant.
Click here to Download Full Paper
AD Publications is a rapidly growing academic publisher in the fields of Engineering, Medical-Health, Environmental Science and Agriculture Research. AD Publications is a registered organization broad-based open access and publishes most exciting researches with respect to the subjects of our journals. The Journals is being indexed and abstracted by all major global current awareness and alerting services.
The organization aims at undertaking, co- coordinating and promoting research and development. It provides professional and academic guidance in the field of basic education, Higher Education as well in the Technical Education. Our Aims is to Promote and support, High Quality basic, Scientific Research and development in fields of Engineering, Medical-Health, Environmental Science and Agriculture Research and to Generate Public awareness, provide advice to scholar’s researchers and communicate research outcomes.
Some Important Links About Research Journal
International Journal
Agriculture Journal
Medical Journal
Environmental Journal
Engineering Journal